The biosynthesis of capsular polysaccharide in Aerobacter aerogenes.
نویسندگان
چکیده
منابع مشابه
The structure of Aerobacter aerogenes capsular polysaccharide.
A capsular polysaccharide produced by Aerobacter aerogenes has been purified and shown to consist of galactose, mannose, and glucuronic acid in a molar ratio of 2: 1: 1, respectively. Partial acid hydrolysis liberated an aldobiouronic acid identified as 2-0-P-D-glucuronosyl-D-mannose. Analysis of capsular polysaccharide by Smith periodate degradation further indicated that all of the galactose ...
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A high molecular weight heteropolysaccharide, composed of glucose, glucuronic acid, N-acetylglucosamine, and mannose in an approximate molar ratio of 1:2:2:5, respectively, was isolated from phage K-2 and from the soluble fraction of phage-infected Aerobacter aerogenes lysates. Treatment of pure phage with 8 M urea at 4 degrees quantitatively solubilizes the bound polysaccharide and capsular po...
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Abstract Background and objectives: important virulence factor for many invasive bacterial pathogens of humans. Escherichia coli offer a model system to study the mechanisms by which capsular polysaccharides are synthesized and exported onto the cell surface of bacteria. Biosynthesis of the E consists of the repeat structure -4) GlcA- (1, 4)-GlcNAc- (1-, requires the KfiA,...
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Strains of Aerobacter aerogenes, A. cloacae and Escherichia coli, including mucoid capsulate, mucoid non-capsulate, smooth and rough variant forms, were grown on defined agar media. The polysaccharide content of the cultures was estimated by the anthrone method and related to the amount of growth as measured by the non-dialysable nitrogen content. Polysaccharide production, as measured by the p...
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Earlier studies have shown1 X5’P to be an obligatory intermediate in the biosynthesis of nucleic acid guanine in Aerobacter aerogenes and related organisms (24). The present paper describes the isolation of X5’P aminase, the enzyme responsible for the conversion of X5’P to G5’P, from extracts of A. aerogenes, and discusses the mechanism of this reaction and its role in purine metabolism. A simi...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 246 1 شماره
صفحات -
تاریخ انتشار 1971